Core B: Cell Culture and Translational Core
The Cell Culture and Translational Core, designated as Core B, is a centralized facility that provides primary alveolar epithelial cells and secondary alveolar epithelial cell lines to each of the projects within the program project. Centralization of the cell culture facilities ensures that a continuous supply of high quality and purity alveolar epithelial cells is available to each of the projects.
The Cell Culture Core B is responsible for the isolation and maintenance of primary cultures of human, rat, and mouse alveolar epithelial type 2 cells, rat alveolar epithelial type I cells, rat and mouse lung fibroblasts, rat and mouse alveolar and peritoneal macrophages, and rat lipofibroblasts. The Core uses standard techniques previously described by our group. The core purchase and propagate human, rat, and mouse alveolar epithelial cell lines. The purity of the cell cultures is verified using a variety of immunological and functional assays.
The Core B also provides rat and mouse precision-cut lung slices. Lung slices are provided using a Leica VT1000 S vibrating blade microtome vibratome and are viable up to 48 hours in culture.
The cell culture facility personnel have extensive experience in the isolation of all the different cell types as well as general cell culture techniques. The consolidation of the cell culture facilities provides an economical means of isolating and culturing lung cells.
Faculty Associated With Core B:
Karen M. Ridge , Core Leader
Emilia Lecuona, Co-Leader
Richard Wunderink, Co-Investigator