Karen M. Ridge, Ph.D.
Research Assistant Professor
Graduate School: University of Illinois at Chicago, Chicago, IL Post-doctoral Fellowship: Michael Reese Hospital, Chicago, IL. 1997-99 Northwestern University, Chicago, IL. 1999-2001 Dr. Ridge’s research interests are in the structure and function of the intermediate filament system in lung epithelial cells. Intermediate filaments (IFs) are composed of large families of proteins that vary in composition from one cell type to another. IFs form elaborate networks that course throughout the cytoplasm and establish connections with both the nuclear and plasma membrane. At the level of the plasma membrane, IFs are involved as cytoskeletal linkages to the desmosomes and hemidesmosomes of epithelial cells. Our approach to studying the IF system involves biochemical, morphological, immunological, cell physiological, and molecular techniques. Our hypothesis is that the IF system forms a continuous network linking the nuclear and cell surfaces, functioning in such diverse activities as the establishment and maintenance of cell shape, organelle movements within the cytoplasm, and signal transduction. Selected Recent Publications: Na Ni and K.M. Ridge.
Mitochondrial reactive oxygen species mediates the disassembly of keratin intermediate filaments in lung epithelial
cells.
J. Cell Biol., in review 1/2006 Wang, H., J. A. Doll, K. Jiang, D. L. Cundiff, J. Czarnecki, M.S. Wilson, K.M. Ridge, and G.A.
Soff Differential Binding of Plasminogen, Plasmin, and Angiostatin4.5 to Cell Surface b-Actin: Implications for Cancer
Mediated Angiogenesis.
Can Res., in review, 2006. Budinger GR, Mutlu GM, Eisenbart J, Fuller AC, Bellmeyer AA, Baker CM, Wilson M, Ridge K, Barrett TA, Lee VY, Chandel NS.
Proapoptotic Bid is required for pulmonary fibrosis.
Proc Natl Acad Sci U S A. 2006 Mar 21;103(12):4604-9. Epub 2006 Mar 14.
PMID: 16537427 [PubMed - in process] K. Lane, P. Debiase, S. Budinger, K.M. Ridge, M.S. Wilson and J.C.R. Jones.
Laminin-6 Fibers Assemble in the Matrix of Type I-like Alveolar Cells in vitro in a β Integrin- and small GTPase
Dependent Manner.,
in press, 2006. Ridge KM, Linz L, Flitney FW, Kuczmarski ER, Chou YH, Omary MB, Sznajder JI, Goldman RD.
Keratin 8 phosphorylation by protein kinase C delta regulates shear stress-mediated disassembly of keratin intermediate filaments in alveolar epithelial cells.
J Biol Chem. 2005 Aug 26;280(34):30400-5. Epub 2005 Jun 22.
PMID: 15972820 [PubMed - indexed for MEDLINE]Myrianthefs PM, Briva A, Lecuona E, Dumasius V, Rutschman DH, Ridge KM, Baltopoulos GJ, Sznajder JI.
Hypocapnic but not metabolic alkalosis impairs alveolar fluid reabsorption.Am J Respir Crit Care Med. 2005 Jun 1;171(11):1267-71. Epub 2005 Mar 11.
PMID: 15764729 [PubMed - indexed for MEDLINE] Azzam ZS, Adir Y, Crespo A, Comellas A, Lecuona E, Dada LA, Krivoy N, Rutschman DH, Sznajder JI, Ridge KM.
Norepinephrine increases alveolar fluid reabsorption and Na,K-ATPase activity.
Am J Respir Crit Care Med. 2004 Oct 1;170(7):730-6. Epub 2004 Jul 15.
PMID: 15256395 [PubMed - indexed for MEDLINE]
Adir Y, Azzam ZS, Lecuona E, Leal S, Pesce L, Dumasius V, Bertorello AM, Factor P, Young JB, Ridge KM, Sznajder JI.
Augmentation of endogenous dopamine production increases lung liquid clearance.
Am J Respir Crit Care Med. 2004 Mar 15;169(6):757-63. Epub 2003 Dec 30.
PMID: 14701706 [PubMed - indexed for MEDLINE]
Sloniewsky DE, Ridge KM, Adir Y, Fries FP, Briva A, Sznajder JI, Sporn PH.
Leukotriene D4 activates alveolar epithelial Na,K-ATPase and increases alveolar fluid clearance.
Am J Respir Crit Care Med. 2004 Feb 1;169(3):407-12. Epub 2003 Oct 24.
PMID: 14578215 [PubMed - indexed for MEDLINE]
Adir Y, Factor P, Dumasius V, Ridge KM, Sznajder JI.
Na,K-ATPase gene transfer increases liquid clearance during ventilation-induced lung injury.
Am J Respir Crit Care Med. 2003 Dec 15;168(12):1445-8. Epub 2003 Aug 28.
PMID: 12947023 [PubMed - indexed for MEDLINE]
Dada LA, Chandel NS, Ridge KM, Pedemonte C, Bertorello AM, Sznajder JI.
Hypoxia-induced endocytosis of Na,K-ATPase in alveolar epithelial cells is mediated by mitochondrial reactive oxygen species and PKC-zeta.
J Clin Invest. 2003 Apr;111(7):1057-64.
PMID: 12671055 [PubMed - indexed for MEDLINE]
Ridge KM, Olivera WG, Saldias F, Azzam Z, Horowitz S, Rutschman DH, Dumasius V, Factor P, Sznajder JI.
Alveolar type 1 cells express the alpha2 Na,K-ATPase, which contributes to lung liquid clearance.
Circ Res. 2003 Mar 7;92(4):453-60. Epub 2003 Jan 30.
PMID: 12600893 [PubMed - indexed for MEDLINE]
Ridge KM, Dada L, Lecuona E, Bertorello AM, Katz AI, Mochly-Rosen D, Sznajder JI.
Dopamine-induced exocytosis of Na,K-ATPase is dependent on activation of protein kinase C-epsilon and -delta.
Mol Biol Cell. 2002 Apr;13(4):1381-9.
PMID: 11950946 [PubMed - indexed for MEDLINE]
Guerrero C, Lecuona E, Pesce L, Ridge KM, Sznajder JI.
Dopamine regulates Na-K-ATPase in alveolar epithelial cells via MAPK-ERK-dependent mechanisms.
Am J Physiol Lung Cell Mol Physiol. 2001 Jul;281(1):L79-85.
PMID: 11404249 [PubMed - indexed for MEDLINE] Pesce L, Guerrero C, Comellas A, Ridge KM, Sznajder JI.
beta-agonists regulate Na,K-ATPase via novel MAPK/ERK andrapamycin-sensitive pathways.
FEBS Lett. 2000 Dec 15;486(3):310-4.
PMID: 11119725 [PubMed - indexed for MEDLINE]
Waters CM, Ridge KM, Sunio G, Venetsanou K, Sznajder JI.
Mechanical stretching of alveolar epithelial cells increases Na(+)-K(+)-ATPase activity.
J Appl Physiol. 1999 Aug;87(2):715-21.
PMID: 10444632 [PubMed - indexed for MEDLINE] |
